Long PCR primers for epitope tagging - where to order? (Dec/17/2007 )
I am doing epitope tagging for the first time, thus here's a simple question. I designed tagging primers for my gene of interest, and with a restriction site, epitope tag and annealing sequence it comes to 63 bp in length. I wonder, can it be ordered from any primer/oligonucleotide supplier? I mean, regular primers are aorund 20bp, and this one is so long - can it be made with no problems by any "primer company"? Thanks.
You should be able to order thess from any oligo synthesizing company. However, because longer primers are harder to make and the end result is less pure than a preparation of shorter oligos, order a larger amount (e.g. 0.2µmol) and get them PAGE or HPLC purified to ensure that all your primers are 63 bp long.
we order long primers with normal desalting, no extra purification. They have worked for us. They are also cheaper than the HPLC or any other purification ones.
You should be able to get them from any oligo company.
Thanks for your answers, I will go ahead and order!