standard curves - real time pcr (Jul/02/2005 )
Should the PCR effiencies of the standard curves be the same as the unknown samples in absolute quantitation and in the relative quantitation based on standard curves?
-indoubt-
Yes, otherwise your quantitation in not vaild.
-pBluescript-
It would be quite messy and complicated since the PCR effiencies have to be the same. Should they have identical PCR effiencies or is that a range of values where they can be in comparing to each other? What kind of quantitation methods do you use for making it easy?
-indoubt-
Hi indoubt,
I am wandering how you could measuring you PCR efficiency of your unknown samples.
As I know, as long as you are using the same primer for both of your standard and unknown samples, the PCR efficiency should be the same.
Thanks
Hadrian
-Hadrian-