How to ressuspend primers for Sybr? - (Jan/22/2008 )
Hello there! I'm new at qRT-PCR and I'll use Sybr green im my experiments. I just have a basic question to start: Should I ressuspend my liofilized primers in TE, as I do with primers for regular PCR? I suppose so, as primers for Sybr have no fluorescence. Anyway, I decide to ask someone first.
Thanks for help and sorry the dumb question
Regards
-pithecia-
I prepare the stock solution in 1X TE, but the working solution in ddH2O because high concentration of salts inhibits PCR.
-merlav-
We resuspend our primers in water. Works fine.
-Madrius-
we also use MilliQ water to dilute primer
-T. reesei-