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How to ressuspend primers for Sybr? - (Jan/22/2008 )

Hello there! I'm new at qRT-PCR and I'll use Sybr green im my experiments. I just have a basic question to start: Should I ressuspend my liofilized primers in TE, as I do with primers for regular PCR? I suppose so, as primers for Sybr have no fluorescence. Anyway, I decide to ask someone first.

Thanks for help and sorry the dumb question

Regards

-pithecia-

I prepare the stock solution in 1X TE, but the working solution in ddH2O because high concentration of salts inhibits PCR.

-merlav-

We resuspend our primers in water. Works fine.

-Madrius-

we also use MilliQ water to dilute primer

-T. reesei-