Primer concentration ? - (Apr/14/2006 )
Hi ! I'm just starting out in PCR. Can anyone pls enlight me on the calculation of primers.............. I've come across literatures stating primer concentrations in pmol while I get the stock in uM. How to convert to pmol? Is there any formula available?
Forgive me for asking fundamental questions.
µM is a concentration, meaning 10 to the power -6 moles/liter. pmol is the quantity, meaning a total quantity of 10 to the power -9 moles.
Compare with this: you have sugar in water at a concentration of 100 grams per liter. how much of this solution would you need to get 10 grams?... Now apply this reasoning to the primer quantity and concentration...
hi this web site is useful : http://www.promega.com/biomath/default.htm