how to add bases to the primer restriction sites - (Feb/05/2008 )

how to add Base pairs from end to primer restion sites. I want to know how to add restriction sequence of EcoR1, XbaI, bamh1 and SacI to the designed primers.i got one paper regarding this addition but these are differeing from NEB catalog,in catalog they given one base for XbaI, he added two to final primer sequence.can anyone please guide me how to do solve this problem.
thanks in advance.
SVRREDDY.
Ph.D student,
UNITUS, ITALY

-ramirddyag-

QUOTE (ramirddyag @ Feb 5 2008, 06:21 AM)

start your primer with 5-6 A's or T's, followed by the restriciton sites one after the other. Stick to the sequence of restriction sites from NEB. Many times sequences are printed wrong in papers.

eg. Here is 6 A's followed by EcorI seq and Xba I seq.

AAA AAA GAA TTC TCT AGA

-scolix-

Thank you very much for your reply. i will follow your guidelines to start my work.

-ramirddyag-