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PCR efficiencies calculation - (Jun/25/2007 )

Hi
I have to calculate PCR amplification efficiencies. Since i have many samples I will use a mix of my cDNAs and serial dilution of this mix. I am using an old ABI RT-qPCR machine and i dont have the software for data analysis. How can I calculate the slope in this case? Is there any available program for this?
Thank you very much.

-elenaj2007-

Program like OpenOffice Calc of MS Excel shoud do it. (I did it once)

You just need to make a graph, where on X axis you put the common logarithm of your relative concentration and on Y axis the Ct value. The program should be able to make a regression line from the points and display the regression equation and correlation ( it should look like y= -3.7626x + 44.288 where 3.7616 is a slope I think).
Then there is the formula to calculate efficiency like 1/(log slope)? I'm not completely sure.

-Trof-

It is explained in ABI Prisms User Bulletin #2. Just google and you got everything you need to know.

Kr├╝mel

-kr├╝melmonster-