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sequencing new "unknown" plasmid - any good SV40 primers out there? (Apr/23/2007 )

Hi,
I have a plasmid that i've got no (real) map, no sequence data, and no RE data on.
But i do know it has an SV40 site. Does anyone have any good primers that are used in the SV40 area?

Ta
Vetticus

-vetticus3-

QUOTE (vetticus3 @ Apr 24 2007, 12:44 PM)
Hi,
I have a plasmid that i've got no (real) map, no sequence data, and no RE data on.
But i do know it has an SV40 site. Does anyone have any good primers that are used in the SV40 area?

Ta
Vetticus


Hi,
Don't know about primer, but here is what I pulled out as SV40 early promoter, found in a number of plasmid:

gatctgcgatctgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca
gttccgcccattctccgccccatcgctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagcta
ttccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaa

Hope it could help

-Almasy-