Protocol for amplification of gene in plasmid directly from transformed e. coli? - (May/27/2007 )

It's possible to PCR the gene out from E coli right? Or I still have to do mini prep? What about colony PCR? (I'm new)

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Yes, when we do screening after transformation for example, we can just pick colonies, resuspend in water and use them as template for PCR without having to do miniprep. But I think for more important exps, it is better to do miniprep.

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There is a thread with extensive replies or opinions from the professional. Just go and search for it.

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