Primer extension - (Oct/12/2001 )
I am having trouble with primer extension. The only product we are getting are bands as if they were primer-dimers. Does anyone have experience with this and what to do to get rid of it. Also we have tried between 10and 20ug Total RNA and I am wondering if this is enough. On a Northern we see a strong signal with 10ug total RNA. tHANKYOU
since primer dimers formed in your experiment, why does you not redesign new one instead of old one? 10-20ug total RNA is appropriate. alternatively you can use mRNA for primer extension.
I am performing primer extension too now but still have no outcome.share your experience when you success.