PCR or Southern? - (Nov/28/2001 )
PCR is more sensitive of course.
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Hello,I would like to know if PCR is more sensitive than southern blotting about transgene detection.Anyone could tell me?thanksmichel
-ragno-
PCR is more sensitive, but in my experience it can be deceptive because it can give false positive results. It can take a while to optimise the PCR method for each particular sequence you are looking for in order to reduce these false results. In my experience a Southern is the only conclusive evidence that I'd be confident with, although doing a PCR on a sample prior to Southern blotting can save a lot of time in selecting out those samples to do the Southern on that are likely positive. I Hope that helps.
-Heather Neufeld-
PCR + Southern hybridization is more sensitive than PCR alone
-Cthulhu-