primers in PCR - primer removed or not (Nov/22/2006 )

hi everybody,

PCR principle is Replication. invivo, after synthesizing new strand, the primer will be removed from the strand and gap will be filled by pol-I 5'-->3' exonuclease activity. is it the same in PCR product also? (i mean, will the primer be removed from the nwe strand after the finishing of its synthesis?)

thankyou.

The in vivo primers are RNA not DNA, while the PCR primers are typically (though not necessarily) DNA. PCR products always incorporate copies of each primer as part of the final double stranded product. This means that modified primers can be used for incorporating unusual bases or modifications in the product, such as 5' biotin labels.

I don't think so. The components of PCR are Buffer, dNTP, (MgCl2), Primer, water and DNA polymearse, so which of them can remove the primer out of new strand? In in vivo condition, there're many enzymes (in this case I think is RNAse H) to degrade primers, but in vitro,it's different

Yep... primers incorporates to amplified product. I'm agree with phague434, this is why you can insert mutations, tagged nucleotides, etc.

agree with phage434, primers will be part of the newly synthesized strand, and that's why enough volume of primers must be added to any pcr reaction...
anyway, there are no nucleases in these reactions and if you assume this, your product will be smaller and smaller each cycle...
check this link (and focus on primers)
http://www.sumanasinc.com/webcontent/anisa...iology/pcr.html