does anyone know real-time methyaltion pcr? - (Aug/21/2004 )

I want to design a pair of primer for the DLC-1 tumor suppressor gene and the probe, I can find the primer sequence according the reference, but how can I design the probe, please help me with it

thanks

Ming

probe design is not a big deal. use your modified DNA as template and follow probe design rules for regular real-time PCR.