Primer design - (Nov/15/2006 )
I plan to design PCR primers to amply my gene of interest about 660 bps in length. Can I rely on a free software tool like Primer3 and oligo. Could anyone give me an opinion based on past experience, which is the best tool that I can use?
Are commercial software any better then free tools like Primer3?
Earlier I used to take primer sequences from published data but I have started using "primer3" and its working absolutely fine.
I use primer 3. It has yet to fail.
because i always have limitations at where to place the primers i always design the primers by myself and then check for the product as well as their melt temp etc by this program:
you have to be careful not to miscopy the primers and stuff like that.
I used primer3 too. But of course I did checked there are other possible dimer formation, blastn (I am doing amplification and need to be specific) =)