Protocol Online logo
Top : Forum Archives: : DNA Methylation, Histone and Chromatin Study

ChIP primer for promoter region of GAPDH - for taqman analysis (Sep/05/2006 )

Hi everyone!

I perform chromatin immunoprecipitation experiments and want to analyse the efficiancy of my specific enrichment by taqman analysis.
Does anyone of you can tell me the sequence of primers/probe that work well in taqman analysis for the promoter region of human house-keeping genes?

I searched the internet but had no luck so far sad.gif

Greetings & many thanks in advance!

-positive thinking-

These are primers from Activemotif chip kit for positive control

GAPDH For: 5´-TACTAGCGGTTTTACGGGCG-3´
GAPDH Rev: 5´-TCGAACAGGAGGAGCAGAGAGCGA-3´
Product size: 166 bp

-pcrman-

Thanks a lot!
I will use them to design a taqman probe!

Greetings smile.gif
_______________________________________________________

QUOTE (pcrman @ Sep 5 2006, 10:07 AM)
These are primers from Activemotif chip kit for positive control

GAPDH For: 5´-TACTAGCGGTTTTACGGGCG-3´
GAPDH Rev: 5´-TCGAACAGGAGGAGCAGAGAGCGA-3´
Product size: 166 bp

-positive thinking-