Protocol Online logo
Top : Forum Archives: : Molecular Biology

PCR - forward, reverse primers - is the reverse primer the "reverse complement" of the sense strand (Mar/27/2006 )

Hi tongue.gif

Am I correct to assume that the
forward primer is an extact copy of the template sense strand,
while the reverse primer is the reverse complement of the template sense strand?

Any info would be much appreciated!!


That would be correct.


QUOTE (pBluescript @ Mar 27 2006, 07:55 AM)
That would be correct.

ok - Thanks a MIll!!!
Appreciated biggrin.gif