product size for real-time PCR primer - (Feb/01/2005 )
I have primers that give product size of 420bp. Can I use them for real-time PCR?
I usually hear that real-time pcr primes should give a product size of 75-150. What is the reason for that?
I'll appreciate any replies.
Thank you very much.
I think the product of 150 bp is set so that the amplification time can be kept to a minimum. Perhaps the signal to DNA amount is also more linear over this range. That said, I have amplified up to 500-600 bp using QPCR before (Qiagen SyBR Green kit) so it can be done.
Thank you for all the answers.
According to the link from littlecell: