Help -designing primers for real time RT-PCR - (Jun/04/2008 )
I want to design a primer pair for PORCINE (pig) model to analyze the gene expression of HSPA2 and several other genes.
1) when I go to nucleotide sequences of these genes it says mRNA linear, but do they correspond to cDNA?? is it that mRNA sequence always refer to cDNA? I am confused, please help with a explanation.
2) If I cant find cDNA sequence but 3 ESTs are available for HSPA2. can I use these sequences to design primers for Ral time RT-PCRs??
Ok I found out that all mRNA sequences in NCBI are actually cDNA sequences!
can anyone give an answer to my second question??