RT-PCR kit - (Dec/14/2006 )

Hi, there,

I will do reverse transcript-PCR using mammalian cells. When I look for the kit, I found too many choices. Do you guys have some suggestion?

Thanks a lot.

-yiwuya-

If you want to do One-Step RT-PCR, I would recommend the Qiagen Kit . It is really nice working and easy to do. But costs a little.
If you want to do it in to steps and just transcripe the RNA in cDNA with a kit, you could try fermentas first-strand-cDNA synthesis kit. It is not so expensive but works also good.

Hope, I could help you a little

-biomaus-

We use Fermentas first strand cDNA synthesis kit. Has worked fine till now and compararively lot cheaper..

-Calvin*-

Most kits or RT enzymes will do for common RNA, that's not extraordinarily long or has high degree of secondary structure.

Check for the cheapest you can get.

-vairus-