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transgenic mouse - identification by PCR (Aug/27/2006 )


I was wondering if anyone has some tips on designing primers for identifying a transgenic mouse?
Would you design them to cross the promoter and the gene of interest, or just the gene of interst, or somewhere else?
Thanks a million,



To genotype transgenic mice I use primers in any region of the original construct (promoter, ORF, or poly A site) that yields the least number of hits when I check their sequence by BLAST.