TAQ polymerase is needed - TAQ polymerase for 60 cycles (Nov/09/2004 )

Hi everyone!

I run TAIL-PCR (asymmetric PCR) and it contains 60 cycles. I tried different TAQ polymerases, but until now no one has worked.
I would thankful if you have any ideas that will work for sure.

Thanks



Youlia

You can add more taq polymerase after 40 cycles, and then continue cycling. It worked for me.