Real-time PCR amplification curve up and down - (May/31/2005 )

my amplification curve (using taqman) is up and down, why?

-mariateresav-

QUOTE (mariateresav @ May 31 2005, 06:19 AM)

my amplification curve (using taqman) is up and down, why?

What do you mean "up and down"? Did u get the log phase?

-Ribosoul-

Dear friend,
Did you mean your level of your reletive fluoresence signal is inconsistence?
Or your Ct value is up and down, changing all the time?

regards
yong

-yongyk-

QUOTE (yongyk @ May 31 2005, 09:34 PM)

Dear friend,
Did you mean your level of your reletive fluoresence signal is inconsistence?
Or your Ct value is up and down, changing all the time?

regards
yong

yes..... I mean that my Ct value change all the time..........

thank

-mariateresav-