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Primer Design - (May/16/2006 )

Hey... I was hoping I could get some help. I am trying to design primers to do RT-PCR using mRNA from mononuclear cells. Essentially I am lookng to sequence the region. However I am having difficulty finding how to design the primers. I tried using primer3 but it is confusing, anyone know of any other software or a tutorial site that might help?

-bayoubelle311-

i like invitrogen's oligoperfect designer: oligoperfect. as far as tutorials, there are some good ones on protocol-online's protocol section, just do a search smile.gif

-llamacita-

QUOTE (bayoubelle311 @ May 16 2006, 12:18 PM)
Hey... I was hoping I could get some help. I am trying to design primers to do RT-PCR using mRNA from mononuclear cells. Essentially I am lookng to sequence the region. However I am having difficulty finding how to design the primers. I tried using primer3 but it is confusing, anyone know of any other software or a tutorial site that might help?



invitrogen`s stuff is good, but the primers suck recently.

-hady-