Primer Design - (May/16/2006 )
Hey... I was hoping I could get some help. I am trying to design primers to do RT-PCR using mRNA from mononuclear cells. Essentially I am lookng to sequence the region. However I am having difficulty finding how to design the primers. I tried using primer3 but it is confusing, anyone know of any other software or a tutorial site that might help?
i like invitrogen's oligoperfect designer: oligoperfect. as far as tutorials, there are some good ones on protocol-online's protocol section, just do a search
invitrogen`s stuff is good, but the primers suck recently.