Curious - different primer synthesis? - (Apr/26/2007 )
Are there other ways to synthesize primers besides standard oligonucleotide synthesis? Can we generate a plasmid and mutate to our desired primers, cut the sites out and purify just our primers? ...or use M13 to generate ssDNA to desired sequences?
If you've got lots of time on your hands, and if your supervisor has lots of money to waste, sure. How were you going to mutate your plasmid, or change the M13 sequence?
Seriously, given the low price of oligosynthesis, why would you?
Heh, I know, it would seem silly, but I was curious if it could be done easily. Just like to be very thorough in my understanding of particular research topics.
A much more exciting prospect is the idea of using oligonucleotide synthesis to produce an entire plasmid. No more cloning work ever again!!!
That day can't come fast enough!
I totally agree, Zouden. I bet the day will come soon. Some group actually did that once in small pieces and then put it together to create an entirely "artificial plasmid" but that takes a lot of work. That would be awesome to do it all at once!