Checking mRNA expression by RT-PCR - (Jul/05/2008 )
I would like to check whether this predicted gene expressed in any kind of cancer tissues. I design primers on two diffreent exons, based on cDNA sequence from UCSC. Unfortunetely, I get nothing for RT-PCR product. My templete cDNA is commercial available, so should be not a problem. How can I confirm whether RT-PCR can workor not? Anybody can guide me or make a suggestion? Thanks.
Well, to test your method, you could either amplify a housekeeping gene or get a sample of tissue where the gene is expressed.
Thanks. I will try.