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how 2 design rev.transPCR primer? - need functioning protocol.... (Dec/03/2008 )

hi fellows,

can anyone tell me how to design fwd + rev primer for reverse transcriptase PCR ?????????
plz explain with example

for eg: i need primer of b-actin


-student in search-

Just google or pubmed search, you can find a pair primer for b-actin. Or you can search to get one.

Or if you want to design yourself, you can use primer3 or primerexpress (ABI), paste your gene sequence, and run the program, you get it.