RFLP PCR amplification - No amplification in PCR (Dec/21/2005 )
I'm doing some RFLP studies with plant DNA. But I could not get any amplification. Only primers are visualise in the gel. Anyone can guess what will be the problem? With the same DNa, Buffer, dNTP and Taq but with random primer I can get amplification.
my guess would be primer-dimer
try different primers, use hot start....
You could also try lowering the annealing temp or reducing the about of primers you add. This may sound silly - but you are sure that yr primer sequence is actually correct right?