RNA polymerase II antibody recommendation? - (Oct/18/2007 )
Hi, everyone, I'm trying to look at RNA polymerase II association with coding region of genes that I'm interested in. Currently I'm using CTD4H8 from upstate, and I'm not very successful in getting enrichement in my coding region, and I know that the mRNA expression is very much induced after treatment, so I should have a induction of association of Polymerase II with coding region of the gene. Could you tell me your experience with RNA polymerase II antibody, and give me some suggestions? Thanks in advance.
I've used sc-899 from Santa Cruz for the N-terminus of Rpb1 and Ab5408 from Abcam for the CTD. Both worked great.
Thanks a lot for your input!