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SNP - How to design the SNP primer (Jul/13/2008 )

Hello everyone,I have a question .Now I am working on molecular marker.I have 2 parents and I have found the difference between them-the SNP indels.But I don't know how to design the PCR primers to discriminant them. Please give me some advice.Thanks a lot.


If you want to do SNP detection by real-time pcr (best: hyb probes or alternatively taqman probes) you can design them with LightCycler design software (Roche) or Primer Design SW (ABI). Alternatively you find help in the PCR Applications Manual - 3rd edition (Roche Applied Science). I always recommend their manuals - they are scientically well done...