BSA concentration (and dilutions) for PCR - (Nov/29/2006 )
Please excuse my lack of math skills but I'm in Public Health and not required to use them often and this problem is hurting my brain (which I need to use for finals).
I have 10mg/ml concentration of BSA that I want to use in my PCR reaction. I know that I need to add 400ng/ul of BSA and my PCR reactions are 20ul. I beleive I would have to to dilute the BSA first. Beyond that, I am stumped (and busy worring about finals). I would appreciate any help/advice. Thank you.
Hi,
You say you need a final concentration of 400 ng/ul, and your volume is 20 ul. This means that you need a total of 8ug BSA in each reaction.
your BSA stock is 10mg/ml, which is equivilant to 10ug/ul. thus, adding 0.8ul of your stock BSA will give you the correct concentration.
I hope I understood the question correctly.
You say you need a final concentration of 400 ng/ul, and your volume is 20 ul. This means that you need a total of 8ug BSA in each reaction.
your BSA stock is 10mg/ml, which is equivilant to 10ug/ul. thus, adding 0.8ul of your stock BSA will give you the correct concentration.
I hope I understood the question correctly.
Thank you for your help. I was on the right track with my calculations, but becasue I work alone, I don't always feel comfortable with my calculations and it's nice to get a second opinion.