primer efficiency in real time PCR - need for a method explanasion (Nov/16/2005 )
hi guys
i wonder if any one can just tell me how to design a primer efficiency experiment
-husam-
http://docs.appliedbiosystems.com/pebiodocs/04303859.pdf
if the link is flaky, go to www.appliedbiosystems.com and do a search for 'user bulletin #2'
this publication outlines exactly what to do and how to perform the calculations, with an example that is taken through the steps
good luck!
-aimikins-