Primers PCR - storage (Aug/09/2007 )

hi,
I inadvertently left my Primers stock at room temp for 12hrs.I always keep it at -20C, but ?I forgot to put it back after aliquoting it.Will these primer stocks be useful.I am worried.

Any suggestions.

Thanks,
NSM

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If the primers are in TE (even if they are in MilliQ), I wouldn't worry too much, but I would give myself a clip over the ears for not looking after reagents properly. The best way to check is a functional test. Take an aliquot and run an expt.

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i wouldn't worry too much it was the primer stock.

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primers are quite stable at rt for such time.
You may write a thick mark to remeber in the future the primer was left at RT but don't worry.

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I dont think it will degrade that quick either.

Why not just try to run a PCR reaction and find out?

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Quite right, the primers are probably okay. Just give yourself a knock on the head, and remember next time to keep your primers in the freezer.

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