SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (Oct/12/2006 )
Hello Everyone,
I am facing an intriging problem. 
FOr the same cDNA prep, SYBR green does not work (HKGs- B2M, B-Actin, RNA pol) and TAqman for the same samples works (B-Actin, 18S). I am quite confused. ALso
in both reactions my -RNA control (where I omitted RNA in the RT step) is also showing amplification.
WHat are your thoughts?
Thanks in Advance 
-Brown girl in the ring-
Oh I forgot to add
The samples that did not amplify for SYBR were all untreated samples, in the same set all of my treated samples amplified. (tHe RNA is from rat mammary tissue). The treated samples also amplified with Taqman (of course 
)...
This is waht is so confusing 
Help!!!!
QUOTE (Brown girl in the ring @ Oct 12 2006, 04:24 PM)
Hello Everyone,
I am facing an intriging problem.
FOr the same cDNA prep, SYBR green does not work (HKGs- B2M, B-Actin, RNA pol) and TAqman for the same samples works (B-Actin, 18S). I am quite confused. ALso
in both reactions my -RNA control (where I omitted RNA in the RT step) is also showing amplification.
WHat are your thoughts?
Thanks in Advance
I am facing an intriging problem.
FOr the same cDNA prep, SYBR green does not work (HKGs- B2M, B-Actin, RNA pol) and TAqman for the same samples works (B-Actin, 18S). I am quite confused. ALso
in both reactions my -RNA control (where I omitted RNA in the RT step) is also showing amplification.
WHat are your thoughts?
Thanks in Advance
-Brown girl in the ring-