designing the sequencing primer - (Nov/17/2005 )
can anyone please help me with the designing the sequencing primer for pCAAGS
You need to look up the original paper on pCAAGS, Niwa et al. 1991 in Gene, which is unfortunately not online. I'd guess there are already primers specified, but if not, they can be easily designed if you know the sequence near the cloning site. There are often M13 forward and reverse primer sites in these older vectors.
thanks for your immediate and promt reply , i looked for this paper but as you said it is not online i coudent find it ,and since i am new to the field of molecular biology, i am lost in this problem, but i will try out your suggestion and try to desgine my self , but if you have any other suggestion please help me