PCR problems - (Jun/27/2005 )
I have some problmes with my PCR.
i want to amplify about 2 Kb, using primers 60mers with 20bp omologous to my plasmid.
I tryed 5 times the PCR, changing H2O, template, PCR program, buffer, protocols... i have also controls but i still have no results.
primers are OK, i checked them
is it possible that the tail of 40bp cause problems in my PCR?
I would think so. Why do you have a 40mer tail?
20 bp of omology with the plasmid and 40mers tails (omologous to another sequence).
ok. after 6 times.... it seems it's working.
i changed templates and program.