PCR problems - (Jun/27/2005 )

I have some problmes with my PCR.
i want to amplify about 2 Kb, using primers 60mers with 20bp omologous to my plasmid.

I tryed 5 times the PCR, changing H2O, template, PCR program, buffer, protocols... i have also controls but i still have no results.
primers are OK, i checked them

is it possible that the tail of 40bp cause problems in my PCR?

yes

20 bp of omology with the plasmid and 40mers tails (omologous to another sequence).

suggestions?

ok. after 6 times.... it seems it's working.

i changed templates and program.