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PCR product dimer (not primer dimer!) - PCR (Apr/12/2006 )

Is it normal to have pcr product dimer?
The product is supposed to be 700bp, but a 1400bp non-specific band showed on the agarose gel...Could it be a dimer?


Try boiling your sample before running on the gel. If it disappears, forget about it. If it doesn't it's probably non specific amplification, most likely of a pseudogene

-John Buckels-

What do you mean "boiling"? Will it degrade the DNA?
And also I do not understand the non specific amplification of pseudogene? If I cut the band with the same size DNAs, how could it to be two distinctively different two bands after gel extraction? sad.gif