problems with PCR long target gene - (Dec/17/2004 )
My target gene is 2.3kb, seems too long to common PCR. with what methods or reagent i can PCR this gene out completely?
Thank you for you reply.
what's your template? genomic DNA or cDNA? I expand recommend high fidelity PCR system from ROCHE.
You can use a long and accurate PCR that easy amplify up to 7 kb. For example BD Clontech provides a kits (Advantage 2 PCR Kit) to perform such PCRs.