AmpliTaq Gold - high fidelity polymerase? - (May/28/2007 )

Dear all,

Do anyone know if AmpliTaq Gold contain a proofread function?

From your experiences, using the AmpliTaq Gold in PCR, what is the max cycle no. can be used if I want to keep the fidelity of my PCR product?

Thanks so much!!!!!

siuchi

Isnt all taq do not have proofreading ability?

Never use such a high fidelity polymerase before.

I normally do not go more than 25 cycles doing high fidelity PCRs. Also addition of BSA is thought to improve the efficiency of high fidelity polymerase reaction.