Old telomerase - PCR elisa kit - can it work? - (Nov/09/2005 )
Lately I've found and dig out a kit for Telomerase PCR ELISA, apparently never used, dated 1998. Someone must have bought it and changed plans later. How do you think - is it still usable?
I know of 7-years old Taq polymerase which was in fact working (I don't know how the efficiency differed, though).
I am asking because my boss is very happy about this discovery and wants to use it, but he's not molecular, so...
Plus: I suspect it will work in some degree, but is it reliable, that is the question...
-Telomerase-
hi
seven years are quite old... But it's interesting to try since you have the kit.
But if it was stored -20° instead of -80° (if recommended) you can throw it away.
-fred_33-
QUOTE (fred_33 @ Nov 9 2005, 07:58 AM)
hi
seven years are quite old... But it's interesting to try since you have the kit.
But if it was stored -20° instead of -80° (if recommended) you can throw it away.
seven years are quite old... But it's interesting to try since you have the kit.
But if it was stored -20° instead of -80° (if recommended) you can throw it away.
It was recommended at -20 and stored in -20, I'd have to check how were the control cell extracts
preserved - liophilised (good) or in solution (bad).
I am afraid though that even if it works, the results will be unreliable.
Thanks anyway
-Telomerase-