Protocol Online logo
Top : Forum Archives: : DNA Methylation, Histone and Chromatin Study

primer design for MSP - How many CpG in the primer? (Sep/28/2005 )

I would like to design some new primers for methylation-specific PCR. How many CpGs should there be in the corresponding DNA? Is one enough? Or is it the more the better?


at bare minimum one C and ideally this is found at the very 3' end of the primer. if you know the methylation status of adjacent CpG's then you can incorporate these into your MSP primers.

Good luck!