primer calculation - concentration (Jul/19/2006 )
what will be the concentration of a primer (100ng/ul) (24 bases) in moles
detail of the calculaltion will be welcome
As missele said:
"Missele Yesterday, 06:01 PM Post #5
hmm, I have a head ache, and I can't find the right result !
1 base is 330 dalton, it means 330g/mol. from 330g you get 1 mol
For a 18-mer, it's quite 18 times : 1 mole is 330*18=5940g
you have 100 ng/µL
100 g is 100/5940=0.016 mol
100 ng is 0.016 nmol
100 ng/µL is 0.016 nmol/µL, or 0.016mmol/L (id est 0.016 mM)"
Therefore it's 0,012mM or 12uM