How to make PCR using the gel solution - (Jun/28/2002 )
Hi, I want to clone a special RAPD band now. Now I plan to cut down the gel with the band, disslove it in some solution (not extract DNA from the gel) and then make a PCR again. But I do not know what kind of solution the gel can be dissolved in and how many the volums the solution will be used in the next PCR . I hope your help. Thank you very much.
In QIAquick Gel Extraction Kit Protocol, the buffer QG can dissolve gel when incubating the gel and QG buffer at 50 degrees for about 10min