primers for Q-PCR - (May/19/2005 )
I am using the program Oligo 6.0 to test a primer pair. When I analyze the parametres, the program gives an error; the melting temperature of the product is much higher than the PCR primers pair one.
Is it very important?
Thanks a lot.
This is expected and not an error, as your product is much longer than your primers and therefore require a higher temp to denature, that is why in your PCR cycle you have a denature step of 95C or thereabouts.