Primer conc - (Nov/12/2007 )
Please help me; I want make 100uM oligonucleotide stock from 329pMol(2ug) (20 mer) supplied (lyophilized in TE).
100 uM it is 100 umol in 1L
or 100 pmol in 1 uL
you should resuspend your 329 pmol in 3.29 uL
that sounds strange. Where am I wrong?
me too......that sounds strange.
I have to admit I have a headache,
but... shouldn't you have more than 329 pmoles ? it's really few.
Usually I order something around 50 nmoles at least.
Please refer page v of the user manual [link: http://www.invitrogen.com/content/sfs/manu...selpizt_man.pdf
I want to make OpIE2 forward & OpIE2 Reverse primer to 100uM stock.
I think missele is right with the calculations.
100uM = 100umol/L = 100nmol/ml = 100pmol/ul
329pmol should be diluted in 3.29ul for 100pmol/ul conc.
As the primers belong to a kit, you should refer to the manual for resuspension. They have optimized protocols.
I'm too busy today to read it, but I guess that there are all details in the manual. usually they are really well down, and you don't need to use your brain, just read and follow instructions