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PCR and DMSO - (Apr/14/2006 )

i want to have some information about the condition of using DMSO in a PCR!!
i want to imrove my inverse PCR results!!

thanks, merci


DMSO may decrease self anneal of primers. Hence by adding it you may decrease non specific amplification. It's admitted generally that 2%DMSO is the max, but i've done 10%DMSO with great results.


Check these references for PCR with DMSO:
Amplifications 5: 16
Biotechniques 10: 58
Gene 140: 1
Nucleic Acids Research 18: 1666