Viral RT PCR - (Mar/20/2006 )

Hello,
I'm currently undergoing a in vivo study of prevalence of RNA viruses from blood,
I have a hard time deciding which RT protocol is best fitted: a hexamer based general RT then amplified by different PCR reactions, or a one step virus specific RT PCR.
Does anybody have experience with amplifying viruses.
Cheers

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We amplify viral RNA from plasma, and we do this by one or two step RT PCR with specific primers.

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Thanks for your message,
Do you reckon the use of hexamer would have an acceptable yield?

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