primer design with intentional mismatch - (Aug/30/2006 )

Hi there,

can anybody give me some sources about primers with an intentional mismatch? normally they are somehwere among the last few bases of the primer at the 3'end. Do they increase the senitivity or specificity? How and why do they do that?

Thanks, have a good time,
Liz.

Hi there,

can anybody give me some sources about primers with an intentional mismatch? normally they are somehwere among the last few bases of the primer at the 3'end. Do they increase the senitivity or specificity? How and why do they do that?

Thanks, have a good time,
Liz.

QUOTE (lizzief @ Aug 30 2006, 02:34 PM)

Hi there,

can anybody give me some sources about primers with an intentional mismatch? normally they are somehwere among the last few bases of the primer at the 3'end. Do they increase the senitivity or specificity? How and why do they do that?

Thanks, have a good time,
Liz.

I think that is because the researcher wants to induce a mutant base.

Hm. the whole thing is about a point mutation detection, and for example jones et al. uses for his ARMS pcr assay a primer which includes the mutated base at the 3'-end and a mismatch at the 3rd base before the 3'-end to "maximize discrimination of the 2 alleles" (cause he runs the mutant specific in the same system like the wild type).
anybody tried something like that before? are there any papers or something in the www anywhere? i cannot find anything

thanks for any help.
Liz