Protocol Online logo
Top : Forum Archives: : Molecular Biology

long PCR for mtDNA - (Jul/03/2001 )

Just buy some of the mixes that various companies sell for this.

They are Taq with a little bit of a proofreading enzyme mixed in, and have names such as Expand or "LA" or "HiFi". Search on a website such as or or

We usually get all our reagents from Sigma just because they work and the price is right.


I would like to have some information about long PCR for the amplification of the total mitochondrial DNA (16-17kb) of mytilus.Thank you.Athanasia