Why using heated-top or add mineral oil in PCR - (Jan/02/2008 )
Still do not get the reason to use heated-top or add mineral oil if without heated-top. I went through internet information. Still very confused!
Heated top prevents condensation that accumulate on the interior cap, thus prevents changing of the buffer concentration. MO covers the liquid layer thus does the similar job.
Using heated top or mineral oil prevents loss of liquid. Changes in salt and buffer composition due to evaporation and condensation, might affect enzyme activity.