Taq PCR and A-tailing - (Nov/08/2005 )
When doing PCR with Taq, some of the products will carry A-tails. I was curious if anyone knew what the percentage of the total PCR product contain the A-tails. I had a look at the fact sheet for the PROMEGA taq but this information was not given. Thanks.
-ML1975-
Check this article, which gives addition rates for a variety of 5' sequences on the primers being used:
Brownstein, M. J., Carpten, J. D., and Smith, J. R. (1996). Modulation of Non-Templated Nucleotide
Addition by Taq DNA Polymerase: Primer Modifications that Facilitate Genotyping. BioTechniques
20, 1004-1010.
-phage434-
QUOTE (phage434 @ Nov 9 2005, 04:17 PM)
Check this article, which gives addition rates for a variety of 5' sequences on the primers being used:
Brownstein, M. J., Carpten, J. D., and Smith, J. R. (1996). Modulation of Non-Templated Nucleotide
Addition by Taq DNA Polymerase: Primer Modifications that Facilitate Genotyping. BioTechniques
20, 1004-1010.
Brownstein, M. J., Carpten, J. D., and Smith, J. R. (1996). Modulation of Non-Templated Nucleotide
Addition by Taq DNA Polymerase: Primer Modifications that Facilitate Genotyping. BioTechniques
20, 1004-1010.
Thanks Phage434, I have had a quick read and it looks exactly like what I needed.
-ML1975-