Protocol Online logo
Top : Forum Archives: : Molecular Biology

Control for primer extension - (Nov/04/2004 )

Hi
Any one plz tell me what control should i take for primer extension of RNA.

-Praveen Bawankar-

For negative control, use no RNA or RNA from cells that don't express your gene.

For positive control, maybe include some gene for which transcription start site is known.

-paulina-

I have looked for a positive control, but I canĀ“t find a gene which transcription start point is known.

-Mone13-